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Figure 3 | Retrovirology

Figure 3

From: Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors

Figure 3

Expression and binding tests of maltose binding and glutathione-S transferase fusion proteins. (A) MBP lysates were bound to amylose resin, eluted with 15 mM maltose, electrophoresed on 10% SDS-PAGE gels, and stained with Coomassie brilliant blue. Lanes 2–4, expression of pmalc2 (empty vector), pmalc2-mIN, and pmalc2-hIN in TB1 cells. For the GST fusions, the lysates were bound to glutathione sepharose, eluted with 10 mM reduced glutathione, electrophoresed on 10% SDS-PAGE gels and stained with Coomassie brilliant blue. Lanes 5–13, representative loads of GST-yeast two hybrid clones: pGEX2TPL, mLEDGF, Fen-1, Enx-1, TFIIE-β, Ku70, ABT1, PRC, and Brd2. (B) Lanes 2–12, GST-yeast-two hybrid clones: AF9, Baz2b, B-ATF, Ankrd49, Znfp38, SF3a3, U2AF26, U5snRNP, KIF3A, Radixin, and Ran bp10. Lane 1 in A and B: Molecular weight marker.

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Retrovirology

ISSN: 1742-4690

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