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Figure 1 | Retrovirology

Figure 1

From: Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors

Figure 1

Expression of DNA binding domain-IN plasmids and control plasmids used in the yeast two-hybrid screens. (A) Lysates from strain CTY10-5d were electrophoresed on 10% SDS-PAGE gels, transferred to PVDF membrane and probed with anti-lexA. Lane 1, pSH2-1 empty vector; lane 2, pSH2-MoMLV IN; lane 3, pSH2-MoMLV IN with 5'six-glycine linker; lane 4, pSH2-HIV-1 IN; lane 5, pSH2-mouse LEDGF; lane 6, pNlexA empty vector; lane 7, MoMLV IN-pNlexA. (B) Lysates from strain SFY526 were electrophoresed on 10% SDS-PAGE gels, transferred to PVDF and probed with anti-GAL4-DB. Lane 1, strain without vector; lane 2, pGBKT7 empty vector; lane 3, pGBKT7-MLV Gag; lane 4, pGBKT7-MoMLV IN; lane 5, pGBKT7-HIV-1 IN; lane 6, pGBKT7-mLEDGF.

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Retrovirology

ISSN: 1742-4690

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