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Table 1 Detection of HTLV-2 sequences in PBMCs from inoculated rabbitsa

From: Human T-cell leukemia virus type 2 post-transcriptional control protein p28 is required for viral infectivity and persistence in vivo

 

Weeks Post Inoculation

Inoculum and Rabbit

0

2

6

8

11b

729.HTLV-2

     

   R27

-

+

+

-

+ (12.0)

   R28

-

+

+

+

+ (8.3)

   R29

-

+

+

+

+ (5.3)

   R30

-

+

+

+

+ (32.8)

   R31

-

+

+

-

+ (10.7)

   R32

-

+

+

+/-

+ (4.2)

729.HTLV-2Δp28

     

   R20

-

+

+/-

+

- (0.2)

   R21

-

+/-

-

-

- (0.1)

   R22

-

+/-

-

-

- (0.3)

   R23

-

+/-

-

-

- (0.3)

   R24

-

+/-

-

-

- (1.1)

   R25

-

-

-

-

- (1.2)

729

     

   R1

-

-

-

-

- (0.1)

   R6

-

-

-

-

- (0.3)

  1. aGenomic DNA was isolated from rabbit PBMCs and subjected to standard PCR (40 cycles) using HTLV-2 specific primers (TRE-pH6-S/TRE-pH6-AS). -, no amplified PCR fragment; +, amplified PCR fragment.
  2. bNumbers in parentheses at wk 11 denote copy number per 1000 cells of rabbit PBMC as determined by real-time RT PCR. Copy numbers in rabbits inoculated with 729.HTLV-2Δp28 at wk 11 were significantly different than 729.HTLV-2 as determined by ANOVA followed by Turkey's test (p<0.00032)