Figure 4
Latent HIV-1 infection in SupT1 T cells. TNFα-induced reactivation of silent HIV-1 proviruses in SupT1 cells was analyzed by determining the percentage of CA-p24 producing cells by intracellular FACS analysis. (A) After a single round infection with the LAI isolate, the culture was split and one culture activated for 24 h with TNFα and the other used as a control. (B) The control culture was maintained for one week and split again into a TNFα treated and control culture. Statistical significance was determined with a two-tailed student's T test (GraphPad Prism).