Figure 7
From: Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+T lymphocytes
Surface expression of CD83, DC-SIGN and MR is affected by ATP. iDCs (5 × 105) were either left untreated or treated for 60 min at 37°C with ATP (400 μM), NL4-3Balenv or ATP and NL4-3Balenv. Next, cells were extensively washed and either stained immediately or cultured for 48 h in complete medium before staining. Cells were stained with antibodies specific for CD83, DC-SIGN, or MR and analyzed by flow cytometry. Results from one representative experiment and flow data acquired after 48 h are presented. a %, Percentage of cells expressing the studied cell surface marker as defined by flow cytometry. Statistical analyses were performed on the results of three independent experiments (b, P < 0.01 ; c, P < 0.05).