Figure 3

HIV-1 is transferred to a lower number of CD4⁺ T cells in presence of ATP. iDCs were either left untreated (mock) or pulsed for 60 min at 37°C with NLHSA-IRES (20 ng of p24/10⁵ cells) in absence or presence of ATP (400 μM). Next, cells were extensively washed to remove input virus and co-cultured for 3 days with autologous CD4⁺ T cells. Cells were then stained for CD3 (FITC labeling) and HSA (R-PE labeling) and analyzed by flow cytometry. The percentage of HSA-expressing cells in the CD3-positive population is shown for three independent experiments.