Figure 1
From: Preclinical evaluation of lime juice as a topical microbicide candidate
Virucidal activity of lime juice. HIV-1BaL (105 TCID50) was exposed to 5% (blue circles) or 10% (red circles) LiJ (diluted using RPMI 10% FBS) for 5 – 120 minutes. This HIV/juice mix was then diluted 1/10 with RPMI 10% and applied to human cervicovaginal explants. Following 2 hours, explants were washed to remove juice and excess virus, and cultured for 10 days with 50% media feeds every 2–3 days. HIV-1 infection was determined by measurement of p24 Ag release into culture supernatants. Data shown are expressed as percentage infection (when compared to an untreated virus control) and represent the mean ± SEM of n = 3 independent tissue donors where each condition was tested in triplicate.