Table 1 Primer sequences and PCR conditions
Target | Primer | Sequences (5'-3') | PCR conditions |
|---|---|---|---|
Gag | For | CCCATAGTGCAGAACATCCA | 50°C, 2 min, 95°C, 2 min, and 95°C, 15 s and 60°C, 30 s, for 50 cycles |
| Â | Rev | GGGCTGAAAGCCTTCTCTTC | Â |
Singly spliced | M669 | GTGTGCCCGTCTGTTGTGTGACTCTGGTAAC | 50°C, 2 min, 95°C, 2 min, and 95°C, 15 s and 60°C, 30 s, for 50 cycles |
| Â | La 23 | GCCTATTCTGCTATGTCGACACC | Â |
β -actin | Actin F | ATGGCCACGGCTGCTTCCAGC | 95°C, 15 s, 55°C, 30 s, 72°C, 15 s for 30 cycles |
| Â | Actin R | CATGGTGGTGCCGCCAGACAG | Â |
GAPDH | GAPDH F | GAGTCAACGGATTTGGTCGT | 95°C, 15 s, 60°C, 30 s, 72°C, 15 s for 30 cycles |
| Â | GAPDH R | TTGATTTTGGAGGGATCTCG | Â |