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Table 3 Zidovudine susceptibility analysis

From: Identification of a novel resistance (E40F) and compensatory (K43E) substitution in HIV-1 reverse transcriptase

Resistance-associated amino acid in RT

40

41

43

184

210

215

219

Average

  

Fold

Consensus B

E

M

K

M

L

T

K

IC50 (nM)

  

Increase

Wild type (HXB2)

.

.

.

.

.

.

.

114

±

10

 

Wild type+K43E

.

.

E

.

.

.

.

90

±

36

1×

M41L+T215Y

.

L

.

.

.

Y

.

1544

±

402

14×

M41L+T215Y+E40F

F

L

.

.

.

Y

.

21307

±

8810

187×

M41L+T215Y+K43E

.

L

E

.

.

Y

.

1556

±

496

14×

M41L+T215Y+E40F+K43E

F

L

E

.

.

Y

.

15350

±

5022

135×

Pat A

F

L

E

V

W

Y

T

14739

±

3105

129×

Pat A-WT40

.

L

E

V

W

Y

T

1596

±

377

14×

Pat A-WT43

F

L

.

V

W

Y

T

13127

±

4582

115×

  1. Pat A-derived virus clone revealed additional amino acid changes including T200I, R211K, V245T, E248D, I293V and E297H
  2. The mean IC50 values of at least two experiments are shown (± standard error of the mean); in the most right column the fold increase compared to the wild type HIV-1 HXB2 reference strain is shown.
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Retrovirology

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