Analysis of galectin-1 expression in WT and mutant Tax-expressing cells. A,B. 293T cells were transfected with 40 μg of the control vector phβPr.1neo, Tax expression vectors (Tax 703, TaxΔ3, Tax M22, and Tax WT) or full-length proviral DNA K30 clone. RT-PCR analyses for galectin-1, Tax and β-actin RNA levels (A) and real-time RT-PCR for galectin-1 RNA levels (B) were conducted on RNA from each transfected conditions. The activated transcription factors for each Tax expression vectors are indicated below panel A. C. CEM-T4 and Sup T1 cell lines were transfected with 20 μg of the control vector pHβPr.1neo or Tax WT expression vector. Total RNA was analyzed by RT-PCR for galectin-1 and β-actin RNA levels. PCR products were separated by electrophoresis on 1.5% agarose gels.