Table 2 Primer sequences for RT-PCR analysis of cytokines.
| Â | Polarity | Sequence | Product size |
|---|---|---|---|
IFN-γ | sense | 5-CATGAAAATCCTGCAGAGCC-3 | 304 bp |
| Â | antisense | 5-GGACAATCTCTTCCCCACCC-3 | Â |
TNF-α | sense | 5-GGCAGGTCTACTTTGGAGTCATTGC-3 | 307 bp |
| Â | antisense | 5-ACATTCGAGGCTCCAGTGAATTC-3 | Â |
TNF-β | sense | 5-TGGCTGGGAACAGGGGAAGGTTGAC-3 | 205 bp |
| Â | antisense | 5-GTGCTTTCTTCTAGAACCCCTTGG-3 | Â |
IL-1α | sense | 5-CTCTAGAGCACCATGCTACAGAC-3 | 308 bp |
| Â | antisense | 5-TGGAATCCAGGGGAAACACTG-3 | Â |
IL-1β | sense | 5-TTGACGGACCCCAAAAGATG-3 | 203 bp |
| Â | antisense | 5-AGAAGGTGCTCATGTAATCA-3 | Â |
IL-6 | sense | 5-GCCAGAGTCCTTCAGAGAGAT-3 | 213 bp |
| Â | antisense | 5-CCGAGTAGATCTCAAAGTGAC-3 | Â |
iNOS | sense | 5-GTCGACCTTCCGAAGTTTCTGGCAGCAGCG-3 | 470 bp |
| Â | antisense | 5-GTCGACGAGCCTCGTGGCTTTGGGCTCCTC-3 | Â |
β-actin | sense | 5-TGTGATGGACTCCGGTGACGG-3 | 198 bp |
| Â | antisense | 5-ACAGCTTCTCTTTGATGTCACGC-3 | Â |