Figure 5

Expression levels of the MuLV protein, CAgag, in ICR-A, R1A and P8A cell lines. (A) Western blot analysis for expression of CAgag in cell lysates (50 μg) using anti-CAgag antibody. β-actin was used as a sample concentration marker. Zpl 2-1 and R1B (12-month-old SAMR1 brain): negative controls; P8B (12-month-old SAMP8 brain) and SC-1-Tf-P8A1 (SC-1 cells infected with P8A1 cell homogenate): positive controls. (B) Densitometry of CAgag and β-actin in the ICR-A, R1A and P8A cell lines. CAgag protein levels were significantly higher in the P8A cell lines than in the R1A cell lines. *statistically significant difference (p < 0.01). (C) Immunofluorescence analysis of CAgag in ICR-A, R1A and P8A cell lines. Expression of CAgag in R1A, P8A and ICR-A cell lines was analyzed using anti-GFAP (green) and anti-CAgag (red) antibodies. DAPI staining (blue fluorescence) was used as a cellular marker.