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Table 2 Production of Gag antigen SIV in dividing and non-dividing CEMx174 cells.

From: Role of the long cytoplasmic domain of the SIV Env glycoprotein in early and late stages of infection

Virus

MTT 1 +aphid1day/+aphid 3 days (OD)

Viability index (fold difference)

p27 ng/ml 2 -aphid.3 days

p27 ng/ml2 +aphid.3 days (x3)3

SIVmac239

0.327/0.106

3

28

17

SIVmac239T

0.302/0.105

2.9

32

41

SIVmac18

0.334/0.104

3.2

10

12

SIVmac18T

0.386/0.117

3.7

19

41

  1. 1MTT assay is described in Methods
  2. 2Amount of Gag p27 antigen in supernatants determined by ELISA described in Methods
  3. 3amount was adjusted in according to results of MTT assay.
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Retrovirology

ISSN: 1742-4690

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