Figure 4
From: Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species
Envelope binding properties of PAR. QT6 cells stably expressing HA-tagged receptors huPAR-1, huPAR-2, muPAR and ratPAR (A) or the chimeric receptors huPAR-2 with L109P mutation and muPAR with P109L mutation (B) were incubated with 1 ml of medium (grey filled) or with the supernatant of 293T containing N-terminal c-myc tagged soluble PERV-A 14/220 SU ENV (bold line). The cells were then immunostained with an anti- human c-myc antibody and a PE conjugated anti-mouse IgG secondary antibody. The histograms show a representative result of at least three independent experiments.