Figure 2
Immunohistochemistry of SIV/SHIV-infected pre-pubertal macaque testis (A-C) and normal adult human testis (D-F). Human testis tissue was used as positive control for antibodies targeting immune cells present in the testis. Formalin-fixed, paraffin-embedded testis tissue was stained with isotype matched control (IgG1, data not shown), a macrophage and dendritic cell marker (DC-SIGN, panel A and D), a myeloid cell marker (CD68, panel B and E) and a dendritic cell marker (Fascin/p55, panels C and F). DAB-positive cells indicated by arrows in sections conterstained with haematoxylin.