Immunofluorescence analysis of transfected HeLa-tat III cells. HeLa-tat III cells were transfected with mutant and wild type proviral DNA constructs. Forty-eight hrs post-transfection, cells were fixed and stained with a mouse anti-CAp24 monoclonal antibody. As a secondary antibody, FITC-conjugated (green) rabbit anti-mouse IgG was used. DAPI (4',6-diamidino-2-phenylindole dihydrochloride) was used to stain cell nuclei. The images in the right column represent an overlay of anti-CAp24 and DAPI stained images.