Figure 5

Safeguarding of CD4⁺ lymphocytes viability. Representative flow cytometry histograms (patient no. 9) characterizing viability of CD4⁺ T cell subset at the end of culture when cells were preincubated one or two days before their polyclonal activation. A gate A was set on the forward-scatter vs side-scatter histogram. As shown on different histograms, gate A corresponded to CD69⁺ CD4⁺ T lymphocytes. The analysis of the 7AAD level expression demonstrated that activated CD4⁺ T lymphocytes were viable cells.