Replication of CQ passaged virus. The replication of CQ passaged or the control passaged 293.10 viruses were tested for their replication in the absence of CQ. CA-p24 or 1 ng/ml was used as input for monitoring replication A) day 37 of passage, B) day 77 of passage, C) day 103 of passage, D) day 147 of passage, E) day 183 of passage and F) day 206 of passage. Standard deviations are depict in all panels. All virus replications were performed in triplicate. G) Determination of TCID50/ml values of passaged viruses in the absence or presence of CQ. Viral infectivity of the viruses passaged in the absence or presence of CQ (days 37, 77, 103, 147, 183 and 206) was measured on CD4+ T-lymphocytes. Standard deviations are depicted.