Figure 5

Env(NYP) is more readily inactivated by treatment with sCD4 than Env(wt). Cells expressing either Env(wt) or Env(NYP) were collected from the culture dish using a non-enzymatic cell dissociation solution and treated with 25 μg/ml sCD4 for 30 min at 37°C. Cells were washed twice to remove free sCD4 and co-incubated with HeLa-CD4/CCR5(wt) cells for 2 hr at 37°C. The results are plotted as percentage of fusion observed for untreated effector cells.