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Table 1 Properties of HIV-1 NC zinc fingers mutants produced by HeLa-P4 cells

From: Intracellular HIV-1 Gag localization is impaired by mutations in the nucleocapsid zinc fingers

Virus total p24 release (S) % of p24 in virions (V) relative levels of gRNA in virions Infectivity
wt 100% 100% 100% +
H23C 37 ± 9 31.5 ± 4.5 10 ± 3 -b
H44C 18.5 ± 9.5 3 ± 1 3.5 ± 1.5 -
ΔZF2 43 ± 6 8.3 ± 0.5 5 ± 1 -
H23CH44C 24.7 ± 12 17 ± 7 4 ± 0 -
ΔZF1ZF2 23.7 ± 10 1 ± 0.1 3.5 ± 0.5 -
ΔNC ~10 0.5 ± 0.1 nd -a
  1. Viral production was assessed by Elisa test: The percentage of CAp24 found in filtered viral supernatant and in virions after ultracentrifugation (V) in comparison to wt (the numbers are representative of at least 2 experiments). The relative level of gRNA in virions was assessed by dot blot. The infectivity was assessed on HeLaP4 relative to the same amount of gRNA. References: (a) by [24]; (b) by [1]. Note that ΔZF1 is described in [1].