Figure 4

HTLV-1 p30 does not modulate apoptosis in 293T cells or Jurkat T-cells. A) p30-expressing Jurkat T-cells or mock infected Jurkat T-cells were treated with camptothecin, etoposide, or TRAIL and assayed for apoptosis induction via Annexin V flow cytometry. Data represents the results of three independent experiments. Although camptothecin, etoposide, and TRAIL induced both cell lines into apoptosis, a differential degree of apoptosis induction was not seen between the two cell lines (nonparametric Wilcoxon rank sum test, p values: camptothecin 0.82, etoposide 0.51, TRAIL 0.13). Standard error bars are indicated. B) 293T cells were transiently transfected with either pME-p30HA or the empty pME-18S vector. Cells were untreated or treated with camptothecin or etoposide. Cell lysates were harvested and 50 μg of lysate was separated by SDS-PAGE. Apoptosis was assayed via immunoblot for the 89 kDa fragment of cleaved PARP. Expression of p30 was verified via immunoblot for HA. Expression of β-actin was verified as a loading control. - cells transfected with empty vector control; + cells transfected with pME-p30HA.