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Table 1 Primer sets for RT PCR

From: Analysis of the contribution of cellular and viral RNA to the packaging of APOBEC3G into HIV-1 virions

Target

Primer Sets

 

forward

reverse

α-tubulin 1)

cacccgtcttcagggcttcttggttt

catttcaccatctggttggctggctc

GAPDH 2)

gaaggtgaaggtcggagtc

gaagatggtgatgggatttc

β-actin 3)

atggatgatgatatcgccgcg

ctagaagcatttgcggtggacg

7SL 3)

gggctgtagtgcgctatgc

cccgggaggtcaccatatt

Vif

gatggcaggtgatgattgtgtgg

ctgtccattcattgtatggc

hY1 4)

ggctggtccgaaggtagtga

aaagactagtcaagtgcagtagtgag

hY3 4)

ggctggtccgagtgcagtg

aaaggctagtcaagtgaagcagtgg

hY4 4)

ggctggtccgagtgcagtg

aaagccagtcaaatttagcagtggg

hY5 4)

agttggtccgagtgttgtggg

aaaacatgcaagctagtcaagcgcg

U1 5)

cctggcaggggagataccatgatcacg

ggggaaagcgcgaacgcagtccccc

U2 5)

cttcttggccttttagctaagatc

ggtgcactgttcctggaggtactgc

U4 5)

gctttgcgcagtggcagtatcg

cagtctccgtagagactgtcaaaaattg

U6 5)

gtgctcgcttcggcagcacatatac

ggaacgcttcacgaatttgcg

  1. 1) Eppendorf cMaster RTplusPCR system (Eppendorf Inc. Westbury, NY)
  2. 2) Funaki et al. 2003 [54]
  3. 3) Onafuwa-Nuga et al. 2006 [28]
  4. 4) Chiu et al 2006 [22]
  5. 5) Giles et al. 2004 [41]
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Retrovirology

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