Persistence of attenuated HIV-1 rev alleles in an epidemiologically linked cohort of long-term survivors infected with nef-deleted virus

Table 1 Subjects, longitudinal blood samples and corresponding laboratory studies.

Subject	Date infected	Date of bloodsample	CD4+ T-cells^a (cells/μl)	Viral load ^b (RNA copies/ml)	HIV-1 progression status^c	No. Rev clones sequenced^d
D36	12/1980.	5/1995	N/A	1400	SP	10
		1/1997	367	3200		10
		7/1999	N/A	BD		10
		4/2001	476	BD		10
C18	8/1983	12/1993	809	N/A	LTNP	10
C64	4/1983	8/1996	925	BD	LTNP	10
		8/1997	805	BD		10
		4/1999	1026	BD		10
		5/2000	875	BD		10
C98	2/1982	10/1995	576	670	SP	10
		2/1997	629	770		10
		11/1999	646	690		10
		5/2001	527	760		10

a; CD4+ T-cells were measured by flow cytometry.
b; Plasma HIV-1 RNA was measured by COBAS Amplicor HIV-1 Monitor Version 1.0 (Roche Molecular Diagnostic Systems, Branchburg, N.J.) prior to July 1999 and Version 1.5 after July 1999. HIV-1 RNA levels < 400 copies/ml (Version 1) or < 50 copies/ml (Version 1.5) were considered below detection.
c; The clinical status of the subjects has been described in detail previously [3, 4, 27].
d; The consensus sequences of the 10 Rev clones sequenced from each time point are shown in Additional file 1.
BD, below detection; N/A, not available; SP, slow progressor; LTNP, long-term nonprogressor.

ISSN: 1742-4690