Figure 2
From: The control of viral infection by tripartite motif proteins and cyclophilin A
A putative mechanism for activity of CypA on HIV-1 infectivity in cells from Old World monkeys. HIV-1 recruits CypA to around 10% of its capsid monomers in newly assembled cores [52, 53]. When the core enters the cytoplasm of a target cell it recruits more CypA, which efficiently catalyses cis/trans isomerisation of the peptide bond at CA G89-P90 [42, 57]. This activity replenishes the cis conformation CA as it is recruited into the restricted complex with TRIM5α. If CypA activity is reduced in target cells, using CypA specific siRNA or by inhibiting CypA activity with CSA, then the OWM TRIM5α cannot interact with the CA, which is mostly in the trans conformation, and infectivity is rescued [59-61]. The isomerisation at CA G89-P90 is represented by squares (trans) changing to circles (cis) on the surface of the capsid.