Figure 5
From: Immunogenicity of the outer domain of a HIV-1 clade C gp120
Purification of ODCN54+-Fc, ODCN54+-Fc(VA) and ODCN54+-His. Protein present in the supernatant of recombinant baculovirus infected insect cells was purified by a combination of lectin and protein A chromatography and analysed by 10% SDS-PAGE. Track M – markers with molecular weights as shown; track 1 – ODCN54+-Fc; track 2 – ODCN54+-Fc(VA) and track 3 – ODCN54+-His. Note the slightly slower migration of the VA mutant as a result of the hydrophobicity change in the loss of two leucines within the Fc coding region. The smear of ODCN54+-His was typical and reflects the high level of glycosylation on a fairly small protein fragment. The yield for all constructs was ~1 mg/L of culture.