Figure 5
Vpx and the proteasome inhibitor MG132, but not Arsenate, display similar effects on the infection of DCs. A) DCs were either infected with Vpx-deficient SIVMAC or HIV-1 LVs (A and B, respectively, at MOI of 0.5 and 5) and treated singularly or in combination with 1 μM arsenic acid (As2O3) and Vpx-VLPs (at MOI equivalents of 0,5 and 2,5). The efficiency of infection was evaluated 72 hrs after by flow cytometry analysis. C) DCs were infected with Vpx containing or deficient SIVMAC LVs (MOI 5) in presence or absence of MG132 (1 μg/ml). The drug was added 30 min prior to infection then left for a total of 7 hrs prior to cell washing and media replacement. DCs were then lysed at 24 hrs post-infection for FL PCR analysis as described in the legend of Fig. 5A. Results are presented as a fold increase in FL DNA for each condition with respect to the amount produced upon infection with Vpx-deficient SIVMAC LVs. D) DCs were similarly infected with a constant amount of HIV-1 LVs in presence or absence of MG132 and 2 different amounts of Vpx-VLPs. Media was replaced 7 hrs post-drug addition and cells analyzed 2 days afterwards by flow cytometry. One representative experiment out of 3 is shown here for each panel.