Figure 2

Vpx allows the accumulation of full length viral DNA following SIV _MAC infection of DCs. DCs were infected with normalized amounts of SIV_MAC LVs containing or not Vpx (MOI of 2). Cell aliquots were harvested at 4 and 24 hrs post-infection and analyzed by semi-quantitative PCR (serial five-fold sample DNA dilutions) using primers that recognized specifically early (MSSS) and late (FL and 2LTRs) products of reverse transcription. The amount of sample added in the PCR reaction decreases from right to left, as represented by triangles: sample amount). Amplification of mitochondrial DNA (mtDNA) was used for normalization. PCR products were transferred onto a nylon membrane and hybridized with ³²P-labelled specific probes prior to phosphor imager analysis and quantification. One representative data set out of 4 independent experiments is shown here.