Volume 3 Supplement 1

2006 International Meeting of The Institute of Human Virology

Open Access

Genetic diversity and high proportion of intersubtype recombinants of HIV-1 in urban Cameroon

  • Ana Machuca1,
  • Shixing Tang1,
  • Jinjie Hu1,
  • Sherwin Lee1,
  • Vutukuri Gupta1,
  • Leopold Zekeng2,
  • Bih Awazi2,
  • Chris Vockley1 and
  • Indira Hewlett1
Retrovirology20063(Suppl 1):P61

https://doi.org/10.1186/1742-4690-3-S1-P61

Published: 21 December 2006

Background

A major characteristic of HIV-1 is its broad genetic diversity, which has significance for HIV transmission, treatment, diagnosis and prevention. We examined the genetic diversity of HIV in blood donors in Cameroon to understand the virus genotype distribution in this population.

Methods

A total of 88 HIV-1 positive samples, collected from blood donors in urban Cameroon in 2002, were genetically characterized in part of HIV-1 env (gp41) and gag (p17) regions through phylogenetic analysis.

Results

All the samples were infected with HIV-1 group M. No group N and O viruses were identified in the samples studied although they are endemic to Cameroon. HIV-1 infections were dominated by circulating recombinant form CRF02_AG (59%) accompanied by additional group M subtypes or CRFs: A, D, F2, G, CRF01_AE, CRF13_cpx and CRF22_01A1. A high percentage (26%) of the samples had discordant subtype designations between gag and env, and appear to be unique intersubtype recombinant forms (URF) with the majority (91%) involving recombination with either CRF01_AE or/and CRF02_AG. URFs containing CRF19 or CRF22 as well Fgag-Genv and Cgag-Aenv are reported. Some samples were not consistently detected by current HIV-1 testing assays.

Conclusion

Our study confirmed the existence of high number of cocirculating HIV-1 subtypes, the high intrasubtype diversity, and the high numbers of possible new recombinant viruses in urban Cameroon. These results highlight the epidemiologic importance of HIV-1 CRFs, especially CRF02_AG.

Authors’ Affiliations

(1)
Center for Biologics Evaluation and Research, Food and Drug Administration
(2)
Mobile Laboratory, Sanitation and Hygiene, Administration of Health

Copyright

© Machuca et al; licensee BioMed Central Ltd. 2006

This article is published under license to BioMed Central Ltd.

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