Skip to main content
  • Poster presentation
  • Open access
  • Published:

A monoclonal antibody against HIV Tat attenuates neurotoxicity via glutamate receptors

HIV dementia is a neurodegenerative disease for which there is no available neuroprotective therapy. Viral proteins, such as Tat, have been implicated as agents of neurotoxicity via multiple mechanisms, including effects on glutamate receptors.

We coincubated HIV-1 Tat protein with a monoclonal antibody against the N-terminal of Tat, in human neuronal cultures. The antibody significantly (P < 0.005) attenuated the neurotoxicity caused by Tat alone, as measured by mitochondrial membrane potential. This protection occurred despite previous observations that peptides from the N-terminal of Tat are non-toxic. We hypothesized that the antibodies may have a more indirect neuroprotective function, preventing excitotoxicity at glutamate receptors. Thus, we coincubated Tat and anti-Tat antibody with NMDA and kainate, in our cultures. The Tat-antibody combination attenuated the toxicity seen with NMDA (p < 0.05). There was some protection against kainate (AMPA) excitotoxicity, but it was not statistically significant (p < 0.2).

The Tat-antiTat antibody complex appears to prevent excitotoxic effects at glutamate receptors, especially NMDA receptors. Host immune responses may influence host susceptibility to the effects of viral proteins, altering HIV complications, such as onset of HIV dementia.

Author information

Authors and Affiliations


Corresponding author

Correspondence to Jeffrey Rumbaugh.

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Cite this article

Rumbaugh, J., Bachani, M., Tikhonov, I. et al. A monoclonal antibody against HIV Tat attenuates neurotoxicity via glutamate receptors. Retrovirology 3 (Suppl 1), P54 (2006).

Download citation

  • Published:

  • DOI: