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  • Open Access

HIV-1 infection in dendritic cells in HAART suppressed patients

  • 1,
  • 1,
  • 1,
  • 1,
  • 1,
  • 1,
  • 5,
  • 4,
  • 2,
  • 3 and
  • 1
Retrovirology20063 (Suppl 1) :P39

https://doi.org/10.1186/1742-4690-3-S1-P39

  • Published:

Keywords

  • Dendritic Cell
  • Viral Load
  • Infected Patient
  • Viral Population
  • Glycosylation Pattern

The different pathways of HIV transmission from dendritic cells to T cells and their possible capacity to harbor the virus, indicates the potential of this group of cells to be a viral reservoir, allowing the virus to evade immune responses. Therefore, we isolated in vivo blood dendritic cells (DCs) from HIV-1 infected patients undergoing HAART. Proviral DNA amplification from DCs was obtained from all infected patients except in patients with non-detectable viral loads. A significant finding was that DCs proviral DNA amplification were obtained from three patients with extremely low viral loads, which resembles the normal viral blips in HAART suppression. This DCs viral infection may be responsible for the replenishment of important reservoirs. DCs' capacity to harbor archaic viral quasispecies was also revealed in two of six patients and those quasispecies were at least as archaic as resting CD4+ T cells viral quasispecies. In addition, the DCs proviral DNA of these two patients presented multiple changes in glycosylation patterns equal to resting CD4+ T cells reservoir. Other important analyses of nucleotide distance and phylogeny revealed a close relation between the viral populations of DCs and resting CD4+ T cells in four HAART failure patients. These data suggest that DCs can act as a short-term reservoir and could be partially responsible for the replenishment of other important reservoirs. Due to the well-documented capacity of DCs to pass the HIV infection to T cells and phylogenetic evidence, we suggest that the infection of DCs in HAART suppression may be partially responsible for the replenishment of the resting CD4+ T cell reservoir.

Authors’ Affiliations

(1)
Molecular Virology Laboratory, Department of Biochemistry, Ponce School of Medicine, Ponce, Puerto Rico
(2)
Department of Pharmacology, Ponce School of Medicine, Ponce, Puerto Rico
(3)
Molecular Genetics Laboratory, Department of Genetics, San Juan Bautista School of Medicine, Caguas, Puerto Rico
(4)
Molecular Biology and Human Diversity Laboratory, Department of Biological Sciences, Florida International University, Miami, Florida, USA
(5)
Clínica Especial de Salud, Juana Díaz, Puerto Rico

Copyright

© Maldonado et al; licensee BioMed Central Ltd. 2006

This article is published under license to BioMed Central Ltd.

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