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Effect of HIV on production of anti-viral factors by HIV-specific CD4+ T cells
Retrovirology volume 3, Article number: P25 (2006)
CD4+ T cells are critical for effective immune responses against HIV, but these cells are targeted by the virus. Here we evaluated the capacity of HIV-specific CD4+ T cells to produce IFN-y and -chemokines and how they were affected by HIV infection.
Materials and methods
HIV gp120-specific CD4+ T cell lines (PS01 and PS02) were generated from PBMCs of HIV+ patients. Intracellular staining and ELISA were used to detect IFN-y, -chemokines, and virus infection.
The CD4+ T cells produced IFN-y and MIP-1a in response to gp120. Also detected were RANTES and MIP-1a, but not MDC (CCL22). The b-chemokines displayed potent inhibitory activities against R5-tropic HIV-1. After the cells were exposed to BX08 (an R5 subtype B HIV-1 primary isolate), the capacity of these cells to produce IFN-y and MIP-1a in response to gp120 was initially comparable with that of the unexposed control. But after 3–4 days of infection, the numbers of cells capable of producing IFN-y and MIP-1a decreased significantly, while the percentage of infected cells reached >80%.
HIV-specific CD4+ T cells are susceptible to HIV. HIV reduces the capacity of the cells to produce IFN-y and b-chemokines. On-going efforts to enhance HIV-specific CD4+ T cell resistance against the virus will be presented.