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Lymphoproliferative responses to HIV-1 Gag p24 antigen and mitogens in HIV-1 subtype CRF01_AE infected Thai patients and HIV-1 seronegative donors

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Background

Lymphocyte proliferation assay has been the standard assay to assess CD4+ T cell function. To determine lymphoproliferative responses (LPR) to HIV-1 p24 and mitogens of PBMC from HIV-1 subtype CRF01_AE infected Thai patients compared with PBMC from HIV-1 seronegative donors.

Methods

Frozen PBMC from 8 HIV-1 infected Thai patients and 8 HIV-seronegative donors were tested and compared for LPR to multiple concentrations of HIV-1 p24 antigen, phytohemagglutinin (PHA), and pokeweed mitogen (PWM). HIV-1 infected patients were grouped by the presence of CD4+ counts > 350 [mean = 483.5 cell/mm3, n = 2 (group A)] or CD4+ counts < 350 [median = 75.5 cells/mm3, n = 6 (group B)]. A stimulation index > 5 was considered a positive LPR.

Results

Both HIV-1 infected patients and HIV-seronegative donors Thai demonstrated positive LPR to PHA and PWM (8/8, 100%). LPR to HIV-1 p24 were seen only patients in group A (100%, 2/2), not in group B (0%, 0/6). This difference was significant (Chi-square test = 11.0, p = 0.0357).

Conclusion

This result suggests that CD4+ T cells in HIV-1 subtype CRF01_AE infected Thai patients with CD4+ counts >350 cells/mm3 have ability to proliferate in response to HIV-1 p24 and indicate they remained the good CD4+ T cell function.

Author information

Correspondence to Thippawan Chuenchitra.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Keywords

  • Lymphocyte Proliferation
  • Standard Assay
  • Stimulation Index
  • Pokeweed Mitogen
  • Lymphocyte Proliferation Assay