Table 1 Primers utilized in these experiments
From: Impaired RNA incorporation and dimerization in live attenuated leader-variants of SIVmac239
Name | Sequence | Locationa |
|---|---|---|
pUΔ39 | 5'-gtcggtacca/caggtaagtgcaacac-3' | +1189-1254 |
pUΔ33 | 5'-gtcggtacca/cggttgcaggtaagtgc-3' | +1189-1249 |
pUΔ19 | 5'-gtcggtacca/gaggaagaggcctccggttgc-3' | + 1189-1238 |
pUΔ14 | 5'-gcacttacctgcaaccg/tcccgctcctcacgcc-3' | +1216-1254 |
pUΔ6 | 5'-gtgttgcacttacctg/gaggcctcttcctctcc-3' | +1203-1249 |
pDΔ60 | 5'-ggagtttctcacgcccatct/cttacctgcaaccggag-3' | +1230-1326 |
pDΔ51 | 5'-ggagtttctcacgcccatctc/gtgttgcacttacctgc-3' | +1238-1326 |
pDΔ12 | 5'-cccttcctggataaaagacagc/gtgttgcacttacctgc-3' | +1238-1288 |
pDΔ19 | 5'-ggagtttctcacgcccatctc/cttcctggataaaagacagc-3' | +1267-1326 |
pDΔ20 | 5'-ccactctatcttattaccc/tatttcttttttgtgttgcacttacctgcaac-3' | +1229-1305 |
pDΔ8 | 5'-cctggataaaagacagctatttctttttt/cttacctgcaaccggagg-3' | +1226-1283 |
Sgag1 | 5'-gcaaccccagttggatccatctcctgt-3' | +1348-1322 |
su5 | 5'-aagctagtgtgtgttcccatctc-3' | +175-197 |
Senf | 5'-ggcttgagctcactctcttgtgag-3' | +8705-8728 |
Sg | 5'-cttccctgacaagacggag-3' | +567-549 |
sg1 | 5'-gaagcatgtagtatgggcag-3' | +627-646 |
sg2 | 5'-ggcactaatggagctaagaccg-3' | +746-725 |
su3 | 5'-ccggaagggatttattacagtg-3' | -518-497 |
su3-1 | 5'-ggctggctatggaaattagtccc-3' | -390-367 |