Figure 4

Infectivity of virus produced in the presence of AdOx in a single round replication assay in MAGI-X4 cells. A) MAGI-X4 cells were infected with HIV-1 produced in AdOx-treated CEM cells normalized to 0.1 ng virion associated RT levels. The cells were infected for 2 h after which the virus was removed. After 48 h, the cells were fixed and developed as described in Materials and Methods. The number of blue foci were counted by light microscopy. B) Virus was obtained 48 h post transfection from HEK293T cells treated once with 10 μM AdOx 6 h post transfection. MAGI X4 cells (dividing or γ-irradiated cells) were infected with HIV-1 normalized to RT activity. The cells were infected in a small volume and fresh media was added after 2h without removing virus. After 48 hr, the cells were fixed and developed as described in Materials and Methods. The number of blue foci were counted by light microscopy. C) MAGI-X4 cells were pretreated with a single dose of 10 μM AdOx at 6 or 24 hours before infection with untreated NL4.3 virus. Cells were infected with virus normalized to RT activity. For all experiments, each infection was performed in duplicate. The experiments were performed at least twice using independent virus stocks. The average result and standard deviation of the mean are shown for experiments in A-C.