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Figure 6 | Retrovirology

Figure 6

From: Human Immunodeficiency Virus-Type 1 LTR DNA contains an intrinsic gene producing antisense RNA and protein products

Figure 6

Antibody to an internal peptide (4041) of HIV antisense proteins detects proteins from HIV-AS-FLAG transfected cells, but not control-transfected cells on western blot analysis. Polyclonal rabbit antisera from rabbits immunized to peptide 4041 was affinity-purified over a 4041 peptide column (Biosource) and then used in immunoblot analysis. Peptide 4041 (+ control, lane 5), peptide 4040 (- control, lane 6), HIV-AS-FLAG transfected HL2/3 cell lysate (lane 3), and FLAG-affinity isolated eluates from HIV-AS-FLAG transfected (lane 2) vs empty vector transfected HL 2/3 cells (lane 1) were separated on Tris-tricine-urea SDS-PAGE, transferred to nitrocellulose membranes and the blots probed with anti-4041 antibody. M, MultiMark multi-colored standard. Molecular weights are in kilodaltons.

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Retrovirology

ISSN: 1742-4690

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