Figure 5
Replication of viral isolates from rapid and slow progressors in PBMC. Panel A shows the replication properties of the last viral isolates (4-month for 2873, 6-month for 2617 and 2669, 8-month for 1449) from four rapid progressors. Panel B shows the replication properties of the first (6-month for 1984, 4-month for 1084) and last viral isolates (48-month for 1984, 1084 and 1690) from slow progressors. The laboratory viral strain SF 128A was used as control. Each 2000 TCID50 viral inoculum was added to 2 × 107 PHA stimulated PBMC from a pool of two HIV-1 seronegative blood donors. Virus titer (TCID50/ml) was measured by infections of TZM-bl cells by viruses harvested from days 1, 5, 9, 13, 17 and 21.