Figure 4

Low Dlg1 expression in IL-2-independent Tax1ΔC cells. (A) CTLL-2 cells (Dlg1-1, Dlg1-3, CAT, LUC) were infected with Tax1-virus (lanes 5–8) or Tax1ΔC (lanes 9–12), and cultured in the absence of IL-2 for more than one month in culture flasks. Expression of Dlg1 (top), Tax1 (middle) and Tubulin (bottom) in parental IL-2-dependent CTLL-2 (lanes 1–4), Tax1-transformed CTLL-2 (lanes 5–8) and Tax1ΔC-transformed CTLL-2 (lane 9–12), was measured by Western blot analysis. (B) Cells were cultured in the absence of IL-2 and counted by trypan blue staining. Data are representative of two independent experiments.