Figure 2

In vitro induction of HIV gene expression in ACH-2 cells, and demethylation of CpGs in the LTRs. A. Induction of HIV mRNA in ACH-2 cell line by TNF-α. Two micrograms of poly (A)+ RNA was analyzed by northern blotting. B. CpG demethylation after HIV induction by TNF-α. Upper panel, Schematic map of the CpG sites in the U3 region of HIV-1 IIIB LTR. The lower panel, results of bisulfite genomic sequencing. Cells are harvested at the time points indicated on the left. No, cells without TNF-α treatment. Each line represents a result of single plasmid clone. Closed and open circles indicate methylated and unmethylated CpG sites, respectively.