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Figure 4 | Retrovirology

Figure 4

From: Effects of prostratin on Cyclin T1/P-TEFb function and the gene expression profile in primary resting CD4+T cells

Figure 4

Effects of prostratin on CDK9 kinase activity. The amount of cell extracts from DMSO and prostratin-treated cells used in immunoprecipitations were adjusted to precipitate equivalent amount of CDK9 using antiserum against CDK9. Immunoprecipitates were subjected to CTD kinase assays to examine relative kinase activities. Products of kinase assay were examined on SDS-polyacrylamide gels, and the CTD substrate hyperphosphorylated form (CTDo) and hypophosphorylated form (CTDa) are shown at the top. PHA-treated cells were used as a positive control. A portion of immunoprecipitates were analyzed in immunoblots shown at the bottom to confirm that equivalent amounts of CDK9 were precipitated; Cyclin T1 levels present in immunoblots were also evaluated by immunoblots.

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Retrovirology

ISSN: 1742-4690

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