Figure 3
Replication and cell killing capacity of dox-inducible viruses in SupT1 cells. (Left) Virus replication of LAI (△), HIV-rtTA (▲), HIV-rtTAΔ6A (○), HIV-rtTAΔ6B (◆) and HIV-rtTA without dox (×) was determined by measuring of the supernatant CA-p24 concentration after infection with virus (20 ng CA-p24) in a 5 mL SupT1 culture. (Right) The number of cells in each culture was determined by a 30 sec time limit FACS analysis. The cell killing capacity of the viruses was determined as the ratio of SupT1 cells present in the infected culture versus the uninfected control culture.