Skip to main content
Figure 4 | Retrovirology

Figure 4

From: The conserved dileucine- and tyrosine-based motifs in MLV and MPMV envelope glycoproteins are both important to regulate a common Env intracellular trafficking

Figure 4

Chimeras bearing the L3S mutation are relocated throughout the endosomal pathway. Forty-eight hours after transfection with the L3S mutant chimeras cDNA, HeLa cells were treated with cycloheximide for 3 hour prior to fixation and permeabilization. A. Co-staining of L3S mutant chimeras and internalized Cy3-conjugated transferrin revealing the early/recycling endosomes.B. Cells were allowed to take up FITC-conjugated dextran for 30 min. Cells were then extensively washed, and dextran was chased for another 30 min prior to fixation and CD25 staining. FITC-dextran thus revealed some late endosomal compartment. C. Co-staining of CD25 chimeras and Lamp1, a protein resident of the lysosomes.

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us