CD25-MLV and CD25-MPMV accumulate in the TGN. Forty-eight hours after transfection with the appropriate chimera cDNA, cells were treated with cycloheximide for 3 hours prior to fixation and staining. A. Co-staining of CD25 chimeras and Mannose 6-phosphate receptor of 46 kDa (MPR46), a protein that accumulates in the TGN at steady state. B. Co-staining of CD25 chimeras and internalized Cy3-conjugated tranferrin revealing the early/recycling endosomes. C. Co-staining of CD25 chimeras and Lamp1, a protein resident of the lysosomes.