Figure 6
From: The virion-associated incoming HIV-1 RNA genome is not targeted by RNA interference
No inhibition of lentiviral transduction in cells transfected with different shRNA plasmids or siRNA. a) Map of the JS1-Nef genome with the positions targeted by the shRNA inhibitors. b) 293T cells were mock transfected (-) or transfected with siNef or plasmids expressing the indicated shRNAs. The cells were subsequently transfected with luciferase reporter constructs containing the target sequences and relative luciferase expression was measured. The mean values obtained in two independent experiments are shown. The control value (-) was set at 100% for each luciferase reporter. c) 293T cells were mock transfected (-) or transfected with the control pBS, siNef or plasmids expressing the indicated shRNA. The cells were subsequently transduced with the JS1-Nef vector. Transduction efficiency was determined by GFP-FACS. The mean values obtained in two independent experiments are shown. The transduction efficiency for the control experiment (-) was set at 100%.