Figure 2

Sequence-specific inhibition of lentiviral production by RNAi. a) Schematic of lentiviral production. When an shNef-expression plasmid is co-transfected during lentiviral vector production, the lentiviral vector RNA genome containing the Nef target (gray box) can be targeted by RNAi (dark arrow). b) Lentiviral vector stocks (JS1, JS1-Nef and JS1-R2) were produced in 293T cells in the absence (-shNef) or presence (+shNef) of an shNef-expression plasmid and were titrated on SupT1 cells. Transduced cells were analyzed by GFP-FACS. The mean values of three independent experiments are shown. The control values (-shNef) were set at 100% for each lentiviral vector.