Figure 3
From: A method to estimate the efficiency of gene expression from an integrated retroviral vector
Estimating the proportion of cells that were puromycin resistant. A series of cultures, each containing 2.1 × 106 cells, with different proportions of puromycin selected cells and untransduced Jurkat cells (as indicated in the legend) were challenged with puromycin. Cell counts were monitored over time. An orderly change can be appreciated, with cultures containing higher proportions of puromycin resistant cells giving higher cell counts at an earlier time point. An equal number of cells from the sample were challenged with puromycin (curve in red). From this it can be estimated that about one in 16000 cells of this sample was puromycin resistant.