Figure 6

Suppression of BRG1 expression by RNAi inhibits HIV-1 replication. Complete sequence of BRG1 wild-type and mutant siRNA used in transfection. Oligos were designed and synthesized using the OligoEngine website [109]. HIV-1 infected cells (ACH₂, OM10.1 and 8E5) were treated with TNF-α (10 ng/ml) for 2 h, washed, and subsequently electroporated (ACH₂, [113]) or Amexa (OM10.1 and 8E5) treated with increasing amounts (1, 5, or 10 μg) of either wild-type (WT) or mutant (mut) BRG1 siRNA. Forty eight hours later, samples were collected and used for p24 gag ELISA. Western blot against BRG1 showed more than 90% decrease in protein levels in WT and not mutant BRG1 siRNA transfect cells [74].