Figure 1

HIV chromatin transcription at various stages of the cell cycle. To obtain synchronized extracts, both eTat and control cells were first treated with hydroxyurea, released and then treated with nocodazole. Extracts were made from 1 h (G₀), 3 h (early G₁), 6 h (G₁/S), 15 h (late S), and 21 h (G₂) post-nocodazole release and used in an in vitro transcription reaction. Released cells (1/10) were processed for FACS profile (data not shown). Two micrograms of chromatin DNA were used in each reaction. The bottom panels are gels of histones from immobilized DNA after transcription stained with Coomasie Blue. Lanes 1–5 and 11–15 are using eTat extract, and lanes 6–10 are using control pCEP4 extract. The left panel contains HIV-LTR-TAR wild-type and HIV-LTR-TAR mutant (TM26), and 200 ng of naked DNA (AdLuc, [55]).