Table 1 Targets, primer and probe sequences and GenBank accession numbers of sequences used for assay design.
From: Transactivation of elements in the human endogenous retrovirus W family by viral infection
Target | Polarity | Sequence (5'-3') | Acc. no. |
|---|---|---|---|
β-actin | Sense | AACCGCGAGAAATCATGTTTG | AY582799 |
Antisense | CAGAGGCGTACAGGGATAGCA | ||
HERV-W env | Sense | CCAATGCATCAGGTGGGTAAC | n/a |
Antisense | GAGGTACCACAGACAAAAAATATTCCT | ||
Syncytin | Sense | GTTAACTTTGTCTCTTCCAGAATCGA | NM_014590 |
Antisense | CATCAGATCGTGGGCTAGCA | ||
Interferon-β | Sense | ACCTCCGAAACTGAAGATCTCCTA | NM_002176 |
Antisense | TGCTGGTTGAAGAATGCTTGA | ||
11q13 gag | Sense | GTTTGCGGCACCAATCTGT | n/a |
Antisense | CGATCTCTGGTATCTCAGGTCAATG | ||
HSV-1 glycoprotein D | Sense | TTTGCGGAATTGTGTACTGGAT | AY155225 |
Antisense | GAGGCGTATGCGCTTTGG | ||
5p13 gag | Sense | CCTGAGGGCCATGACTAAAGAG | n/a |
Antisense | CCGCCTTAGGCCCAGAGT | ||
Seg. 8 A/WSN/33 | Sense | CAGCACTCTCGGTCTGGACAT | U13683 |
Antisense | TCCTTCAGAATCCGCTCCACTA | ||
HERV-W gag | Sense | TCAGGTCAACAATAGGATGACAACA | n/a |
Antisense | CAATGAGGGTCTACACTGGGAACT | ||
HERV-W 3q26.32 gag ORF, MGB-probe | 6-FAM-CCTGTGGGAGTTGTT-MGB | AF156961 |