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Figure 2 | Retrovirology

Figure 2

From: Intracytoplasmic maturation of the human immunodeficiency virus type 1 reverse transcription complexes determines their capacity to integrate into chromatin

Figure 2

Quantitative analysis of nuclear translocation of HIV-1 RTCs in synchronized cells. A. Cell cycle distribution of control, non-synchronized HeLa cells (upper panel), and cells pre-treated with 2 mM thymidine was measured by flow cytometric analysis before spinoculation (middle panel) and 5 h after spinoculation (lower panel). Percentage of cells at different phases of the cell cycle was counted using CellQuest software. B,C. Nuclear translocation of HIV-1 RTCs. HIV-1 DNA was purified from cytoplasmic and nuclear HIV-1 complexes 5 h after infection of synchronized and non-synchronized HeLa cells. Triplicate samples were analyzed by real-time PCR with primers specific for early and late HIV-1 DNA by measuring SYBR Green fluorescence. Values are means ± SD. Panel B shows percentage of nRTC DNA relative to DNA from cRTCs. Panel C represents percentage of late DNA from nRTCs relative to early nRTC DNA.

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Retrovirology

ISSN: 1742-4690

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