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Figure 3 | Retrovirology

Figure 3

From: Induction of the HIV-1 Tat co-factor cyclin T1 during monocyte differentiation is required for the regulated expression of a large portion of cellular mRNAs

Figure 3

Knockdown of cyclin T1 inhibits Tat transactivation of HIV-1 proviral expression. (A) Non-transduced parental MM6 cells (MM6) or pool of MM6 cells expressing a shRNA against cyclin T1 (shRNA-T1) or a control shRNA (shRNA-Con) were infected with either a NL4-3-Luc (Tat+) HIV-1 luciferase reporter virus or a NL4-3-Luc-Tat- (Tat-) virus encoding a mutated Tat protein. Cell lysates were prepared 48 hours post-infection and analyzed for luciferase activity using equal amounts of protein. Luciferase expressions in extracts infected with the shRNA-Cyc T1 lentiviral vector were assigned an arbitrary value of 1.0 unit and other values are shown relative to this. A representative experiment of this experimental design is shown. (B) MM6 cells were infected with either a Tat+ virus or a Tat- virus. After three days, they were either left uninfected or infected with lentivial vectors expressing a shRNA against Cyclin T1 or a control shRNA. Cell extracts were prepared five days post-infection and assayed for luciferase expression. A representative experiment of this experimental design is shown.

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Retrovirology

ISSN: 1742-4690

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